Figure 5.

K_i determination of compound 1 and mode of inhibition with regard to template/primer substrate. Reaction velocities were measured at different template/primer concentrations (0.05, 0.075, 0.15 and 0.2 µM plotted as closed diamonds, open diamonds, closed squares and open squares, respectively) with the UTP fixed at 25 µM. Dixon and Cornish-Bowden plots of the reciprocal velocity with compound 1 at concentrations of 0, 15, 30 and 60 µM. Compound 1 displayed a mixed mode of inhibition towards template/primer, with a major competitive component (K_i competitive = 26 µM; intercept on the Dixon plot above the x-axis) and a minor uncompetitive component (K_i uncompetitive = 105 µM; intercept extrapolated from the Cornish-Bowden plot below the x-axis).