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Kinetic analysis of 2-CysPrx WT and variants. Representative data are shown for WT. Linear plot of the integrated Dalziel rate equation for a two-substrate reaction. Activity of 2-CysPrx was determined with 75 μM hydrogen peroxide and varying concentrations of E. coli Trx (6 μM, open circles; 8 μM, filled circles; 10 μM, filled squares; 12 μM, open squares) as described in Materials and methods. (B) Secondary Dalziel plot. The data are summarized in Table 4.
