Table 1.

2-CysPrx variants generated and characterized in this study

2-CysPrx variant	Mimicked 2-CysPrx conformation
Wild type (WT)	Redox-variable conformation
C54D	Hyperoxidized form
T92D	Phosphorylated form
C54S	Reduced form, interaction with Trx-like proteins
C176S	Reduced form with putative thiol oxidase function
ΔC (–15 aa)	Peroxidase with a loss of sensitivity to hyperoxidation
C54DC176K	Oxidized form
F84R	Reduced aggregation capacity, increased sensitivity to hyperoxidation
WT, N-6×His	Enhanced flexibility by limited steric constraints