Table 2.
Analysis of CD spectra recorded from reduced and oxidized 2-CysPrx
Data are means±SD of four independent experiments. 2-Cys Prx variants were analysed under non-reducing and reducing conditions. Numbers show the percentage of the respective structures.
| WT | C54D | C54S | C176S | T92D | F84R | C54D/ C176K | ΔC | |
|---|---|---|---|---|---|---|---|---|
| Non-reducing | ||||||||
| Helix | 38±10 | 57±5 | 51±4 | 38±5 | 38±4 | 41±3 | 34±7 | 80±6 |
| β-sheet | 11±3 | 0±0 | 0±0 | 14±8 | 21±5 | 19±2 | 21±7 | 0±0 |
| Turn | 27±3 | 29±3 | 39±2 | 30±3 | 25±3 | 22±4 | 25±1 | 2±2 |
| Random | 24±4 | 14±3 | 10±2 | 18±1 | 16±2 | 17±1 | 19±2 | 18±4 |
| Reducing | ||||||||
| Helix | 28±4 | 42±0 | 29±2 | 33±1 | 33±9 | 32±2 | 31±8 | 18±3 |
| β-sheet | 0±0 | 2±2 | 0±0 | 0±0 | 6±6 | 0±0 | 2±3 | 61±9 |
| Turn | 36±3 | 40±6 | 39±7 | 41±4 | 37±6 | 37±4 | 37±4 | 6±6 |
| Random | 36±6 | 16±4 | 32±9 | 27±5 | 24±6 | 31±7 | 30±10 | 15±6 |