Fig. 2.

Gene structure of OsbZIP58, and grain size and weight of the osbzip58 mutants and CLs. The CLs are transformants containing a wild-type OsbZIP58 gene in the osbzip58-1 mutant background. (A) Diagram of the OsbZIP58 gene structure and T-DNA insertion positions in osbzip58-1 (PFG_1B-15317.R) and osbzip58-2 (PFG_3A-09093.R). Exons are shown as black boxes. Both mutants had a T-DNA insert in the first intron. Primers used in the genotype analysis are indicated by black arrows. GE0279 and GE0301 are gene-specific primers, and GE0295 is the T-DNA specific-primer. (B–E) Grain weight (B), grain length (C), grain width (D), and grain thickness (E). Fifty seeds were analysed for seed size, and data are presented as means ±SD. The 1000-grain weight was determined by counting ten replicates of 100-grain samples independently on an electronic balance. Data are shown as mean ±sd. Two-tailed unpaired tests indicate significant differences in 1000-grain weight and seed size. **P <0.01.