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. 2004 Jan 22;32(2):465–476. doi: 10.1093/nar/gkh191

Figure 5.

Figure 5

SDS–PAGE analysis under reducing or non-reducing conditions of the P.abyssi TrmI protein demonstrates the existence of intersubunit disulphide bridges. Before loading, protein samples corresponding to the WT TrmI protein (lanes 1 and 6), C196S mutant (lanes 2 and 7), C233S mutant (lanes 3 and 8) and C196S+C233S double mutant (lanes 4 and 9) were incubated in the presence (lanes 6–9) or absence (lanes 1–4) of 100 mM β-mercaptoethanol for 5 min at 100°C. The molecular mass markers (Pharmacia-Biotech) were loaded into lane 5.