Figure 5.

Localization of suSLBP during the first cell cycle. Unfertilized eggs (A) and fertilized eggs 30 (B), 45 (C), 60 (D), 75 (E), 90 (F) and 105 min (G) after fertilization were fixed and suSLBP detected by immunofluorescence using affinity-purified SLBP. Pronuclear fusion had occurred at 30 min, the cells had entered mitosis by 90 min and were in metaphase at 105 min. suSLBP is concentrated in the egg pronucleus and remains in the zygotic nucleus during S phase. At mitosis, the suSLBP is dispersed throughout the zygote.