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. 2004 Jan 29;32(2):598–610. doi: 10.1093/nar/gkh195

Figure 1.

Figure 1

Dnmt3a binds to Ubc9, PIAS1 and PIASxα in a yeast two-hybrid screen. Full-length Dnmt3a was fused to the GAL4-DBD and used as bait in a yeast two-hybrid screen against a human fetal liver cDNA library in AH109 yeast cells under high stringency selection conditions. Clones scoring positive for growth on selective media were confirmed using a beta-galactosidase assay in Y187 yeast cells, then sequenced. The identity of two representative, independently isolated clones is shown below the graph of beta-galactosidase activity (as relative units). The positive control (+) is the interaction between p53 and the SV40 T antigen, the negative control (–) is the interaction between p53 and lamin B, and ‘bait only’ is the GAL4-Dnmt3a construct alone under the appropriate selection conditions. Expression of full-length Dnmt3a in AH109 cells was confirmed by western blotting (data not shown). Values are the mean of three independent experiments and the error bar is the standard deviation from the mean.