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. Author manuscript; available in PMC: 2014 Jun 14.
Published in final edited form as: Biochem Biophys Res Commun. 2013 May 17;435(4):671–677. doi: 10.1016/j.bbrc.2013.05.041

Fig. 1.

Fig. 1

Detection of a 35 kDa PGC-1α isoform by different antibodies, and down-regulation of the protein by PGC-1α siRNAs. A) Total protein extracts of SH-SY5Y cells were analyzed by Western blotting with anti-PGC-1α antibodies. B) Purified mitochondria and the remaining cytosolic fraction including nucleus were analyzed by Western blotting with P-120, anti-HSP60, anti-Histone H3, and anti-GAPDH antibodies. C) HEK cells were transiently transfected with scrambled siRNA (lane 1) or two separate siRNAs against PGC-1α (lanes 2 and 3), followed by Western blot analysis with P-120 and actin antibodies.