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. Author manuscript; available in PMC: 2013 Aug 5.
Published in final edited form as: Vaccine. 2011 Dec 4;30(5):922–930. doi: 10.1016/j.vaccine.2011.11.089

Fig. 6.

Fig. 6

Dissection of the CD4bs specificities of serum antibodies. Sera were serially diluted (starting at 1:50) and incubated on plates for 1 h. The indicated mAbs (diluted to 10 μg/ml) were then added to the plates and these incubated for a further 1 h. MAb binding was quantified using AP-conjugated anti-human F(ab’)2-specific secondary antibody (Jackson ImmunoResearch). Sera from animals immunized with Q105N inhibited the binding of mAbs b12, b13 and VRC03 to a greater extent than gp120wt animals, suggesting that the epitopes of the serum antibodies overlap most with the epitopes of these CD4bs mAbs. Error bars denote the signal ranges from replicate wells.