Fig. 7. Serum anti-gliadin antibodies in nude mice after adoptive transfer of gliadin-presensitized CD4+CD45RBlowCD25− T cells.
Serum levels of anti-gliadin IgA and IgG were determined in 2 groups of nude mouse recipients (gliadin/gluten, gliadin/gfd), as well as in C57BL/6 control mice raised on a gluten-free diet (C57BL/6-gfd) or normal (gluten-containing) chow (C57BL/6-gluten; each n=8). Differences for anti-gliadin IgG and IgA between gliadin/gluten and every other group were highly significant (***p<0.001). Levels of anti-gliadin IgG1 and IgG2c were determined in sera positive for anti-gliadin IgG (i.e. in the gliadin/gluten group only).