Table 4.
Secondary structure analysis: a comparison of resilin-like polypeptides
| Resilin-like polypeptide and sequence motif | Technique | Helices (%) | Strands (%) | Turns (%) | Unordered (%) (or random coil) | Beta sheet (%) | PPII (%) |
|---|---|---|---|---|---|---|---|
| An 16a (AQTPSSQYGAP) | CD | 5 | – | 11 | 64 | 10 | 10 |
| Dros 16b (GGRPSDSYGAPGGGN) | CD | 5.3 | – | 11.8 | 45.7 | 27.3 | 9.9 |
| Rec1 resilina (GGRPSDSYGAPGGGN, but irregular)c | CD | 2 | – | 11 | 58 | 18 | 11 |
| Full-length Drosophila Resilin (two motifs)d | FTIR | 11.9 | 15.3 | 29.1 | 43.8 | – | – |
| CD | 9.8 | 16.1 | 17.5 | 41.6 | – | – | |
| Crosslinked Drosophila resilin (two motifs)d | FTIR | 14.2 | 19.9 | 24.4 | 41.4 | – | – |
| CD | 9.3 | 15.7 | 17.9 | 42.7 | – | – |
a
Data reproduced from [229] with permission from The American Chemical Society, copyright 2009
b
Reported in supplementary material from Lyons et al. [229]
c
Sequence derived from first exon of CG15920 gene; therefore, it contains irregularities, but consensus repeat GGRPSDSYGAPGGGN
d
Sequence derived from complete CG15920 gene; contains two consensus repeat motifs: GGRPSDSYGAPGGGN and GYSGGRPGGQDLG [187] with permission from The American Chemical Society, copyright 2009