Table 1.

Summary of assays used to characterize and compound structures of salicylidene acylhydrazide. All IC₅₀ values were determined by light emission from the luciferase gene fused to the YopE promoter in Y. pseudotubercuclosis²⁶.

Compound	Regulation	Secretion/Translocation	T3SS IC50	Infection	Other Pathways	Ref
1 INP-0007, D8	C trachomatis: Down-regulates transcription of late-cycle virulence genes	C. trachomatis: Prevents IncA secretion during infection S. typhimurium Y. pseudo-tuberculosis Y. pseudo-tuberculosis: Prevents YopH translocation into HeLa	~15 μM ~5 μM*	C. trachomatis: Inhibits replication in HeLa S. typhimurium: Reduces hemolysis of erythrocytes, invasion of HeLa, and immune response in bovine ligatcd loop model Y. pseudo-tuberculosis: Inhibits invasion of HeLa	Y. pseudo-tuberculosis: Inhibits motility	21, 26, 31, 35, 38
2 1NP-0010, D9, ME0052	C. pneumoniae, S. typhimurium: Down-regulates transcription of some virulence genes EHEC: Down-regulates transcription of LEE but not iron-related genes	EHEC: Inhibits Tir and EspD secretion S. typhimurium	~25 μM	C trachomatis: Inhibits replication in mammalian cell lines S. typhimurium: Inhibits invasion of MDCK cells and replication in macrophages Y. pseudo-tuberculosis: Reduces macrophage cytotoxicity		27, 32, 26
3 INP-0031, ME0055	EHEC: Down-regulates transcription of LEE but not iron-related genes and up-regulates flagellum expression	EHEC: Inhibits Tir and EspD secretion	~25 μM	EHEC: Reduces number of A/E lesions on bovine epithelial cells		27
4 INP-0400, D1	S. flexneri: Affects needle complex assembly	S. typhimurium S. flexneri		C. trachomatis: Inhibits replication in HeLa and McCoy cells S. typhimurium: Reduces HeLa cytotoxicity S. flexneri: Reduces invasion of HeLa and macrophage cytotoxicity		33, 34, 36, 39
5 INP-0403, D4	S. typhimurium: Down-regulates transcription of invC and hilD but not hilA or effectors	S. typhimurium	~25 μM	S. typhimurium: Reduces hemolysis of erythrocytes, invasion of HeLa, and immune response in bovine ligatcd loop model	S. typhimurium: Effects reversed with exogenous iron	35, 36, 37

^*IC₅₀ value was determined by fluorescence detection from the carboxypeptidase G2-SopE2 reporter construct in S. typhimurium³⁸.