Table 2.

Summary of assays used to characterize and compound structures of 2-imino-5-arylidene thiazolidinone T3SS inhibitors. IC₅₀ values are reported based on the inhibition of SipA secretion as determining by Western blot.

Compound	Regulation	Secretion/Translocation	T3SS IC50	Infection	Other Pathways	Ref
1 TTS29	S. typhimurium: Inhibits needle complex assembly, No effect on general transcription	S. typhimurium Y. enterocolitica F. tularensis	83 μM	P. syringae: Prevents HR response in tobacco leaves S. typhimurium: Reduces macrophage cytotoxicity	S. typhimurium: No effect on flagellar system or motility P. aeruginosa: Inhibits T4-dependent motility & T2S	41, 42, 43
2 TTS29-analog	S. typhimurium: No effect on general transcription	S. typhimurium:	3 μM	S. typhimurium: Reduces macrophage cytotoxicity	P. aeruginosa: Inhibits T2S	41, 42, 43
3		S. typhimurium: Inhibits secretion of SipA	5 μM			43