Table 2. Charge and chemical properties of the ultimate and the penultimate amino acid residues in the nascent polypeptide chain with the observed read-through efficiencies.

Position –2		Position –1		Read-through efficiency (%)
Nature	Amino acid	Nature	Amino acid
Polar	Thr	Polar	Gln	16
Basic	Lys	Polar	Gln	15
Acidic	Asp	Polar	Gln	14
Polar	Gln	Polar	Gln	10
Acidic	Asp	Polar	Thr	5
Polar	Tyr	Polar	Ser	5
Non-polar	Pro	Polar	Ser	4
Non-polar	Pro	Polar	Tyr	2
Acidic	Asp	Acidic	Glu	12
Polar	Thr	Acidic	Glu	11
Basic	His	Acidic	Glu	9
Non-polar	Gly	Acidic	Glu	8
Polar	Thr	Acidic	Asp	4
Basic	Arg	Basic	Lys	7
Polar	Thr	Basic	Lys	6
Acidic	Asp	Basic	Lys	5
Basic	His	Non-polar	Ile	11
Non-polar	Pro	Non-polar	Ile	6
Acidic	Glu	Non-polar	Ile	3
Acidic	Glu	Non-polar	Phe	1