Table 3. Influence of ribosomal P site tRNAs on translational read-through.

Nucleotide sequence					Read-through efficiency (%)
–2	–1	Stop	+1	+2	pFL44La	tRNAUUGGln1	tRNACUGGln1*	tRNACUGGln2	tRNAUUGGln2*
ACA	CAA	TGA	CAC	TTA	8	9	8	8	6
ACA	CAG	TGA	CAC	TTA	2	3	3	2	3

Each experiment was done at least five times with a variability of ∼20%.

^aThe empty pFL44L vector.