Table 5.
Summary of drug screening protocols against Leishmania
| Type of screening |
Analyses type | Need for substrates/dyes |
Parasite stage | Applicability to any parasite strain/host cell |
Host cell | Species | Reference |
|---|---|---|---|---|---|---|---|
| HTS | Resazurin sodium salt (Sigma, St. Louis, MO, USA) | Yes | Promastigote | Yes/no | L. major | Siqueira-Neto et al., 2010 | |
| Draq5 (DNA marker) | Intracellular amastigote | Yes/yes | THP1 | L. major/L. donovani | |||
| HTS | CellTiter-Glo (Promega, Madison, WI, USA) (ATP-bioluminescence) | Yes | Promastigote | Yes/no | L. donovani | De Muylder et al., 2011 | |
| DAPI (DNA marker) | Intracellular amastigote | Yes/yes | THP1 | ||||
| CellTiter-Glo (Promega, Madison, WI, USA) | Axenic amastigote | Yes/no | |||||
| HTS | CellTiter Blue Reagent | Yes | Promastigote | Yes/no | Leishmania sp. | Sharlow et al., 2010 | |
| MTS | RFP fluorescence | No | Intracellular amastigote | Yes/yes | Murine macrophages | L. amazonensis |