Skip to main content
. 2004 Jan 23;32(2):502–510. doi: 10.1093/nar/gkh202

Figure 4.

Figure 4

The RT domain does not account for the difference in insertion lengths of L1.2A versus L1RP. Plotted are the percentages of G418R HeLa clones that are positive for the 3 kb ruler PCR for the wild-type elements L1RP and L1.2A and the RT swapped elements (see Materials and Methods) L1RP with L1.2A-RT and L1.2A with L1RP-RT. None of the L1.2A+L1RP-RT clones was positive in the 3 kb PCR. Shown below the graph are the nRFs, using the retrotransposition frequency of the least active element, L1.2A, as a basis for comparison. The absolute retrotransposition frequency of L1.2A in this experiment was 1/2113. The numbers of clones typed for each element are as follows: L1RP (48 clones), L1RP+L1.2RT (47 clones), L1.2A+L1RP-RT (32 clones) and L1.2A (37 clones). All 164 clones were positive for the 1 kb insertion product.