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. 2004 Jan 23;32(2):502–510. doi: 10.1093/nar/gkh202

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Copyright © 2004 Oxford University Press

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Reciprocal effects of ORF2 amino acids 1220 and 1259 on insertion length and retrotransposition frequency in L1_RP and L1.2A. Plotted are the percentages of G418^R HeLa clones that are positive for the 2 and the 3 kb ruler PCRs for the wild-type elements L1_RP and L1.2A and various permutations at positions 1220 and 1259 in ORF2. L1.2B is identical to L1.2A with I1220 and S1259 (see Fig. 2). Shown below the graph are the nRFs, using the retrotransposition frequency of the least active element, L1.2A, as a basis for comparison. The absolute retrotransposition frequency of L1.2A in this experiment was 1/838. The numbers of clones typed for each element are as follows: L1_RP (46 clones), RP M1220 (24 clones), RP L1259 (24 clones), RP M1220 L1259 (21 clones), L1.2B (19 clones), L1.2A S1259 (20 clones), L1.2A I1220 (17 clones) and L1.2A (54 clones). All 225 clones were positive for the 1 kb insertion product.