Table 2.

Genotype-3a peptides identified in spontaneous resolvers

Protein	Amino acid	3a Peptide sequence*	Patients	1b Peptide sequence	Outcome	Defining CD4:CD8 T cell subsets
E2	610–625	LTPRCMVDYPYRLWHY	109	LTPRCLVDYPYRLWHY	SR	NA
	696–712	LIHLHQNIVDVQYLYGV	568	LIHLHQNIVDVQYLYGI	SR	CD8†
	702–719	NIVDVQYLYGVGSGMVGW	861	NIVDVQYLYGVGSAFVSF	SR	CD8†
NS3 protease	1198–1213	KALQFIPVETLSTQAR	626	KAVDFVPVESMETTMR	SR	CD4
	1288–1305	GNRTVTTGAKLTYSTYGK	109	GVRTITTGAPVTYSTYGK	SR	NA
NS3 helicase	1423–1440	AYYRGLDVSVIPTAGDVV	884	AYYRGLDVSVIPTIGDVV	SR	CD4
NS4b	1805–1822	TSPLTTNQTMFFNILGGW	806	TSPLTTQSTLLFNILGGW	SR	NA
NS5b	2548–2565	NQIRSVWEDLLEDTTTPI	113	NHIHSVWKDLLEDTVTPI	SR	CD4
	2603–2618	KRALYDVIQKLSIETM	884	KMALYDVVSTLPQVVM	SR	CD4
	2893–2908	IIERLHGLSAFTLHSY	113	IIERLHGLSAFSLHSY	SR	CD4

^*Genoype 3a peptide sequence identified as T cell targets in patients with resolved infection. Sequence differences from genotype-1b peptides is shown in bold.

^†CD4:CD8 T cell subset analysis assessed using ICS assays.

CD4:CD8 T cell subset analysis was assessed using PBMC in IFN-γ ELISpot assays following CD8 depletion.

ICS, intracellular cytokine stains; IFN, interferon; PBMC, peripheral blood mononuclear cells.