Table 1.

Primers for cloning 3′ UTRs. Sequences for 3′UTRs of the indicated human genes were obtained from the National Center for Biotechnology Information website. Primers were designed to include the entire UTR beginning just downstream of the stop codon and extending to the site of poly(A) addition. With the exception of the MyD88 mutated clone, a second set of primers was used on PCR products to generate an XhoI site or an EagI site at the 5′ end of the sense or antisense primers, respectively.

Gene (accession #)	Sense primer	Antisense primer
MyD88 (NM_002468)	5′-CCTGCACCAAATCTTGGTTCTGG ACTCG-3′	5′-CAAGGTAGAATATTATTTATTATT ATAAACTCAGGATGCAAGATATATT CCAGG-3′
MyD88 mutated	5′-CCCAATGTACCAGCCCTTATAC CTCTAATGAAGCACAGAGAGAGG-3′	5′-CCTCTCTCTGTGCTTCATTAGAGG TATAAGGGCTGGTACATTGGG-3′
TLR4 (NM_138554)	5′-GGTAAATCATGGAATCCAGAA GGAACAGTGGG-3′	5′-CATAACTTTTATTACAATATA TTATTATAACTATTCAATTTTATTGTTAGT- TATTGTTAATCTCT-3′
IRAK1 (NM_008363)	5′-AACCTGAAAAGAGCCAGGGACC TGAAGAAA-3′	5′-TCAGAGTAATCACCCCCAAAACA AGTGGGG-3′
TRAF6 (NM_004620.3)	5′-TTTTGAAGACTTTCGACTTGCC CTCACTTGCTC-3′	5′-TTTTCGGCCGAACACTTAAA CAAGTATTATTCAACAG-3′
TAB2 (NM_015093.3)	5′-GCCAAATGGCCCTGTATCTTC TCTAAAACCA-3′	5′-CCACATTAGAATATAAGTTTTTTA ATTTTTATAAATAACTTATCTGTTACAAA- GATAGTT-3′