ISS-ODN enhances the Th1-type antibody response in conjugate{OVA-ISS} or lip{LLO,OVA,ISS}-immunized mice. Two weeks after the second immunization, sera were collected and analyzed for anti-OVA IgG1 or IgG2a antibodies using ELISA. (A) Each bar represents geometric mean titers ± standard error of the mean (GMT ± SEM) for each group (n=10). (B) Each bar represents the average of the IgG2a:IgG1 ratio ± S.D. for each group, which was calculated from the ratio of each individual animal (n=10). If no titer was observed, the lowest dilution tested, 600, was reported.