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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Proteomics. 2013 Feb;13(0):10.1002/pmic.201200333. doi: 10.1002/pmic.201200333

Figure 1.

Figure 1

Experimental workflow of analysis of cell-surface sialoglycoproteins using click chemistry. (1) Metabolic labeling of cells with peracetylated azidomannose (AC4ManNAz). (2) Chemoselective conjugation of azido sugars with a biotinylated alkyne capture reagent via Cu (I)-catalyzed click chemistry. (3) Lysis of labeled cells. (4) Affinity purification using streptavidin (SAv) resins. (5) Elution of captured sialoglycoproteins. (6) SDS-PAGE separation of sialoglycoproteins. (7) Isolation of gel slices and subsequent digestion and release of peptides. (8) Analysis of peptides by LC-MS/MS. (9) Bioinformatic analysis.