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. 2004 Jan 23;32(2):477–487. doi: 10.1093/nar/gkh214

Figure 7.

Figure 7

Intracellular localization of mMUTYH proteins. (A) Electron microscopic immunocytochemistry. Thin sections were prepared from fixed wild-type CCE28 cells, MUTYH-null YDK15 cells, mMUTYHα-expressing YDKα cells, and mMUTYHβ-expressing YDKβ cells. MUTYH signals were examined by electron microscopic immunocytochemistry, using anti-hMUTYH. Nuclear and mitochondrial structures are shown with blue and red dotted lines, respectively. Bars: 1 μm. (B) The distribution of MUTYH signals in nuclear and mitochondrial region determined by electron microscopic immunocytochemistry. Gold signals present in nuclear and mitochondrial regions as seen in (A), from 10 sections of each sample were counted, and the number of signals in nuclear or mitochondrial region per section is shown in a box-and-whisker-plot. Each plot shows a box with ends at quartiles, and the statistical median as a horizontal line in the box. The whiskers extend to the farthest points that are not outliers (circles). Both in nuclei and mitochondria, the number of gold signals detected in CCE28, YDKα and YDKβ cells were significantly higher than those in YDK cells (Mann–Whitney U-test, P ≤ 0.0002).