Table 2.
Thermal denaturation temperatures (Tm's) of duplexes between ON4–ON6 and complementary DNA/RNA targets.a
| Tm [ΔTm]/°C | |||
|---|---|---|---|
| ON | Sequence | +DNA | +RNA |
| ON4a | 3'-CAc TtT aCG | 43.5 [+12.5] | 50.0 [+21.5] |
| ON4b | 3'-CAC YTY ACG | 34.5 [+3.5] | 22.5 [−6.0] |
| ON4c | 3'-CAc YtY aCG | nt | 26.5 [−2.0]b |
| ON5a | 3'-CAc TcT aCG | 48.5 [+12.5] | 61.0 [+23.5] |
| ON5b | 3'-CAC YCY ACG | 39.0 [+3.0] | 27.0 [−10.5] |
| ON5c | 3'-CAc YcY aCG | 37.0 [+1.0] | 46.5 [+9.0] |
| ON6a | 3'-CAc TgT aCG | 50.0 [+13.0] | 56.5 [+23.0] |
| ON6b | 3'-CAC YGY ACG | 47.0 [+10.0] | 29.0 [−4.5] |
| ON6c | 3'-CAc YgY aCG | 31.0 [−6.0] | 37.5 [+4.0] |
a
Unmodified reference duplexes display the following Tm's (DNA/RNA): 31.0/28.5 °C (ON4 ref), 36.0/37.5 °C (ON5 ref), and 37.0/33.5 °C (ON6 ref). “nt” denotes no transition. For experimental conditions and definitions, see Table 1.
b
Broad transition. Error of Tm value estimated at ±3.0 °C.