Table 3.
Pyrene absorption maxima for ON1–ON6 and the corresponding duplexes with DNA and RNA targets.a
| λmax [Δλ] (nm) | ||||
|---|---|---|---|---|
| ON | Sequence | SSP | +DNA | +RNA |
| ON1b | 5'-GTG AYA TGC | 348 | 352 [+4] | 352 [+4] |
| ON2b | 3'-CAC YAY ACG | 349 | 352 [+3] | 352 [+3] |
| ON4b | 3'-CAC YTY ACG | 349 | 352 [+3] | 351 [+2] |
| ON5b | 3'-CAC YCY ACG | 349 | 352 [+3] | 350 [+1] |
| ON6b | 3'-CAC YGY ACG | 352 | 352 [±0] | 352 [±0] |
| ON1c | 5'-GTG aYa TGC | 347 | 351 [+4] | 343 [−6] |
| ON2c | 3'-CAC YaY ACG | 348 | 352 [+4] | 351 [+3] |
| ON3c | 3'-CAc YaY aCG | 344 | 351 [+7] | 342 [−2] |
| ON4c | 3'-CAc YtY aCG | 346 | 345 [−1] | 343 [−3] |
| ON5c | 3'-CAc YcY aCG | 348 | 352 [+4] | 342 [−6] |
| ON6c | 3'-CAc YgY aCG | 346 | 352 [+6] | 343 [−3] |
a
Δλ = shift in absorption maximum relative to single-stranded probe (SSP). T = 5 °C; each strand used at 1.0 µM concentration in Tm buffer.