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. 2001 Jun;12(6):1699–1709. doi: 10.1091/mbc.12.6.1699

Figure 2.

Figure 2

Codistribution of syntaxin 6 with a marker of ISGs but not MSGs. The distribution of endogenous SgII (A and E), p18 (B and H), and syntaxin 6 (D and G) in PC12/PC2 cells is shown by double labeling in confocal images, with the use of the anti-SgII antibody 175, the anti-p18 antibody 6B1/3, and anti-syntaxin 6 antibodies (D, mAb; G, polyclonal antibody), respectively. Superimposed images (C, F, and I) demonstrate the overlapping distribution of the syntaxin 6 only with SgII in TGN and ISGs. Full-length SgII, recognized by 175, appears mainly in the perinuclear region containing Golgi membranes and early ISGs (A), whereas p18 staining is found mainly distributed in maturing ISGs and MSGs distributed throughout the cytoplasm (B). Syntaxin 6 immunostaining can be found in the Golgi apparatus (D), where it colocalizes with SgII (E and F). In addition to the perinuclear Golgi staining, a punctate syntaxin 6 staining appears over the entire cell (D and G), which does not colocalize with p18 (H and I). Selected images from three independent experiments are shown.