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. 2004 Jan 29;32(2):611–622. doi: 10.1093/nar/gkh223

Figure 1.

Figure 1

Analysis of the transcription start site of the YB-1 gene by primer extension assay. (A) Primer extension assay. Hybridization of the primer extension was performed with 5′-labeled oligonucleotide and 1 µg poly(A)+ RNA of each cell line. The markers shown on the left are end-labeled HinfI fragments of φ-X174 DNA. Black asterisks (*) indicate transcription initiation start sites. The 5′ furthest transcription initiation start site is indicated as +1 and the first AUG codon is indicated at +331. The arrow indicates the position of the primer that was used for extension. The primer hybridizes the sense strand between nucleotides +317 and +337 of the YB-1 gene. (B) Schematic distribution of the transcription initiation site in the YB-1 5′-UTR.