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. 2004 Jan 29;32(2):611–622. doi: 10.1093/nar/gkh223

Figure 5.

Figure 5

Identification of the YB-1 5′-UTR binding domain in YB-1. (A) Schematic illustration of the GST–YB-1 deletion mutants used in this study. CSD indicates the cold shock domain. (B) REMSA assay. The indicated amount of each GST–YB-1 deletion mutant and GST was incubated with 32P-labeled YB-1 5′-UTR RNA at 25°C for 15 min. An aliquot of 2 µg YB-1-specific antibody (Ab) was added to lanes 11–15. An arrow indicates the YB-1/YB-1 5′-UTR RNA complex and a double-headed arrow indicates supershifted complexes. YB-1 mutant/YB-1 5′-UTR RNA probe complexes were separated using 4% native polyacrylamide gels.