Figure 4.

H2a is partially concentrated in a juxtanuclear region in some untreated cells. Cells expressing H2a were fixed and permeabilized, and double label immunofluorescence was performed with the use of anti-H2 carboxyterminal antibodies and Cy3-conjugated goat anti-rabbit IgG (A and C) and mouse anti-BiP antibodies with FITC-conjugated goat-anti-mouse IgG (B and D). A and B show a cell representative of the general population. C and D show a cell with an H2a immunofluorescence pattern representing ∼5% of the cells. The white arrows show regions with higher H2a concentration and not BiP and black outlined arrows show regions with higher BiP concentration and not H2a. Bar = 10 μm.