Figure 3.

Identification of αCTD residues important for activation of p_E by CII in vitro. Single-round in vitro transcription experiments were performed using linear template DNA containing p_E and p_oop, CII, and RNAP reconstituted with hexahistidine-tagged α derivatives containing alanine substitutions at the positions indicated. The activities of purified RNAPs were normalized at the p_oop promoter. The efficiency of transcription from p_E in the presence of each reconstituted mutant RNAP is shown below the corresponding lane from a typical transcription gel. Values (with standard deviation) are expressed as percentages of the transcript yield obtained with wild-type RNAP.