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. 2013 Jun 5;33(23):9846–9854. doi: 10.1523/JNEUROSCI.0451-13.2013

Figure 1.

Figure 1.

Time requirements for synaptic output from MBN subsets for the retrieval of appetitive olfactory memory. A, All flies were conditioned using an appetitive unconditioned stimulus at 24°C and shifted to 32°C 10 min before a retrieval test. A synaptic blockade imposed by the expression and activation of shits in the α′/β′ MBNs significantly reduced memory expression from 15 min to 3 h after training (Mann–Whitney pairwise comparisons, p ≤ 0.0022). No significant difference was observed during retrieval of memory from 9 to 24 h after training (Mann–Whitney pairwise comparisons, p ≥ 0.2403). No significant difference was observed between permissive and restrictive temperatures for control flies carrying only the c305a-gal4 element (Mann–Whitney pairwise comparisons, p ≥ 0.5887). B, Synaptic blockade of γ MBNs significantly reduced appetitive olfactory memory expression from 15 min to 3 h after training (Mann–Whitney pairwise comparisons, p ≤ 0.0411). No significant difference was observed during retrieval of memory from 9 to 24 h after training (Mann–Whitney pairwise comparisons, p ≥ 0.3315). No significant difference was observed between permissive and restrictive temperatures for control flies carrying only the 1471-gal4 element (Mann–Whitney pairwise comparisons, p ≥ 0.4696). C, Synaptic blockade of α/β MBNs significantly reduced appetitive olfactory memory expression from 15 min to 24 h after training (Mann–Whitney pairwise comparisons, p ≤ 0.045). No significant difference was observed between permissive and restrictive temperatures for control flies carrying only the c739-gal4 element (Mann–Whitney pairwise comparisons, p ≥ 0.1727). D, Synaptic blockade using shits of α/β and γ MBNs eliminated appetitive olfactory memory expression from 15 min to 24 h after training (Mann–Whitney pairwise comparisons, p ≤ 0.0304). The performance of 247-gal4/uas-shits flies at restrictive temperature at all times tested was not significantly different from zero (Wilcoxon signed rank tests, p ≥ 0.0938). No significant difference was observed between permissive and restrictive temperatures for control flies carrying only the 247-gal4 element (Mann–Whitney pairwise comparisons, p ≥ 0.1087). E, No significant difference was observed between permissive and restrictive temperatures for control flies carrying only the uas-shits element (Mann–Whitney pairwise comparisons, p ≥ 0.0916).