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. Author manuscript; available in PMC: 2013 Aug 5.
Published in final edited form as: Biochem Pharmacol. 2011 Aug 17;82(11):1562–1571. doi: 10.1016/j.bcp.2011.08.002

Figure 2. Accumulation of mitoxantrone in MDA-MB-231/R cells in the absence or presence of 5 μM test compounds.

Figure 2

MDA-MB-231/R cells (106cells/ml) were incubated in the absence (control) or presence of 5μM test compound in serum-free RPMI medium for 15 min, 37°C, followed by addition of MX (3 μM) for 30 min. The cells were then washed with ice-cold PBS and resuspended in PBS for the determination of MX on the flow cytometer as described in Methods. 10μM FTC was used a positive control. The MX accumulation in MDA-MB-231/R cells was calculated as a percentage of control (0.1% DMSO) as described previously (Sim et al., 2008). Data points are expressed as mean and error bars represent SD for n = 3 - 4 independent determinations. Statistical difference (*p < 0.05) between MX accumulation in control and treated groups were analyzed using one-way ANOVA analysis followed by Dunnett post-hoc test.