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RT–PCR analyses of IFN-γ up-regulated TrpRS isoforms in HUVECs. Cells were treated with or without IFN-γ (100 U/ml) for 24 h. Total RNAs were isolated and converted to cDNA. PCR was performed with cDNA (prepared as described in Materials and Methods and diluted 1:100) and analyzed by 1.2% agarose gel electrophoresis. Under these conditions, the differences between the levels of TrpRS mRNA isoforms could be seen. GAPDH is an internal control.
