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. 2004 Feb 3;32(2):791–802. doi: 10.1093/nar/gkh248

Figure 2.

Figure 2

TEF-1-mediated transactivation of MCAT-dependent reporter constructs in HEK 293 (A, B, E and F) and C2C12 muscle cells (C and D) depends on VITO-1. HEK 293 cells and C2C12 cells were co-transfected with the reporter construct pTA-LUC-4×MCAT (A–D) and pTA-LUC-1×MCAT (E and F) and pCS2-based expression vectors for TEF-1 and VITO-1, either alone or in combination. The results of three different experimental series each consisting of five independent transfections are shown. Luciferase activities in relative light units (RLU) (A, C and E). Luciferase activities displayed as fold stimulation of pTA-LUC-4×MCAT (A–D) or pTA-LUC-1×MCAT (E and F) background activities. Neither VITO-1 nor TEF-1 alone are sufficient for efficient transactivation of pTA-LUC-4×MCAT or pTA-LUC-1×MCAT while a combination of both leads to an increase in luciferase activity.