Skip to main content
. 2004 Feb 3;32(2):791–802. doi: 10.1093/nar/gkh248

Figure 7.

Figure 7

Knockdown of VITO-1 expression attenuates differentiation of C2C12 muscle cells. C2C12 myoblasts were co-transfected with pSUPER and pEGFP-C2 (A and E), pSUPER-Vito-1 and pEGFP-C2 (B, C, F and G) and pSUPER-Myogenin and pEGFP-C2 (D and H). Cells were examined for EGFP fluorescence to visualize transfected cells (E–H) and stained with a Myogenin antibody (G) (detected with a rhodamine coupled secondary antibody) or inspected by phase contrast microscopy (A–D). Note that transfected cells expressing VITO-1 siRNA (green) are negative for Myogenin (red). Knockdown of VITO-1 and Myogenin expression led to an inhibition of myotube formation while differentiation of C2C12 cells occurred normally after transfection with pSUPER.