Table 3.
Predicted metabolic responses to weak organic acid treatments
|
Fluxa |
Reference |
Treatment |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| |
|
Acetate |
Benzoate |
Propionate |
Sorbate |
||||
| Exp.b | Exp. | Sim. | Exp. | Sim. | Exp. | Sim. | Exp. | Sim. | |
| Glucose |
6.09 |
12.23 |
12.31 |
12.26 |
12.11 |
12.92 |
12.67 |
12.12 |
11.95 |
| CO2 |
10.45 |
22.54 |
22.58 |
21.80 |
21.67 |
24.09 |
22.89 |
20.76 |
21.55 |
| Ethanol |
9.60 |
21.23 |
21.39 |
20.61 |
20.72 |
21.60 |
21.79 |
20.18 |
20.63 |
| Glycerol |
0.79 |
0.54 |
1.56 |
0.96 |
1.43 |
1.00 |
1.56 |
0.83 |
1.28 |
| Lactate |
0.05 |
0.09 |
0.06 |
0.10 |
0.06 |
0.11 |
0.07 |
0.09 |
0.06 |
| Acetate |
0.02 |
−0.57 |
−0.58 |
0.08 |
0.11 |
0.03 |
0.12 |
0.02 |
0.11 |
| C-recov. |
99.41 |
95.14 |
98.71 |
95.68 |
98.60 |
96.48 |
99.24 |
93.47 |
98.91 |
| Yield | 0.09 | 0.05 | 0.04 | 0.05 | 0.05 | 0.04 | 0.04 | 0.05 | 0.05 |
aFluxes from Glucose to Acetate correspond to exchange fluxes in mmol·g-1·h-1; C-recov. denotes the percentage of carbon recovered in the measured exchange fluxes and biomass (we attributed the lost carbon to pyruvate secretion); Yield denotes the production of biomass per unit of consumed substrate (g·g-1).
b Experimental (Exp.) data taken from Abbott et al. [24]. Simulated data are denoted by Sim.