Figure 1.

Outline of cell proliferation experiment. Diagrammatic representation of the 3 in vitro culture conditions applied to NHBE cells and analyzed at the gene expression level (2 hours, 4 hours, 6 hours, and 8 hours after the washing step). From t0 to t1, the cells were cultured in normal growth medium (GM) for 24 hours. PD-0332991 inhibitor (INH, final concentration 1 mM) was then added to the GM at t1 for 32 hours (B) or for 24 hours (C). The control group (A) was cultured in GM and no inhibitor was added. At t2 (after 48 hours in GM), a washing step was done for all 3 conditions. The cells were then cultured in GM (A and C) or in GM plus INH (B).