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. 2013 Aug 5;8(8):e70968. doi: 10.1371/journal.pone.0070968

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© 2013 Lee et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Retinal-induced CD4⁺CD25⁺ Tregs showed >96% purity on flow cytometry. (B) Tregs generated were characterized by positive expression of intracellular Foxp3, CTLA-4, and surface expression of the indicated markers (PD-1, GITR, ICAM-1, CD44, ICOS) in the gated T-cell populations. Also, they showed weak positive surface staining for CD62L and CD103. The percentages indicate numbers of double-positive cells.