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. 2004 Feb 3;32(2):784–790. doi: 10.1093/nar/gkh256

Figure 3.

Figure 3

An inverse relationship between –1 PRF efficiencies and mRNA half-lives. (A) The high efficiency UUUUUUA frameshift signal in pDJ274 promotes an ∼6-fold increase in –1 PRF efficiency and decreases the half-life of the PGK1 reporter from 8 min to 5.75 ± 0.5 min. (B) The GGGCCCA slippery site decreases –1 PRF by ∼11-fold as compared with wild-type. The half-life of the PGK1 reporter mRNA produced by pJD273 is increased to ∼20 min. (C) The half-life of the wild-type –1 PRF signal-containing reporter (pJD269) is 8 min in the absence of drug. Addition of anisomycin (4 µg/ml) to cells harboring pJD269 results in partial stabilization of the PKG1 reporter so that its half-life is 13.5 ± 1.5 min. mRNAs were analyzed as described in Figure 1.