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. 2004 Feb 3;32(2):742–748. doi: 10.1093/nar/gkh257

Figure 1.

Figure 1

Pipeline description. A new integrated and automated strategy for obtaining and administrating data from high-throughput investigations of cDNAs. Input for the described pipeline are FL-cDNAs extracted from large-scale generated cDNAs. Verified ORFs are cloned into expression vectors to be used in high-throughput experimental assays. Additionally, all identified ORFs undergo an exhaustive bioinformatics analysis by running automated tasks (DomainSweep, 2DSweep and ProtSweep). These tasks are part of the W3H task framework, which allows the integration of heterogeneous applications to create tailor-made analysis flows. Both computational and experimental results are integrated into a relational database (‘Core Database’). The core database consists of several single databases, which allow the researcher to cross-check different protein features in silico by simply executing appropriate SQL queries via web browsers or clients.