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A schematic representation of siRNA selection and validation for a set of under-characterized genes. (A) N-terminal, full-length fusion proteins are generated and plasmids are co-transfected with three unique siRNAs corresponding to the gene target and a GFP control siRNA. Protein levels are analyzed by western blot. (B) In a parallel experiment, cells are transfected with siRNAs only and the mRNA levels for the endogenous genes are analyzed by RT–PCR.
