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Normalized thermal denaturation profiles of the SMN1 and SMN2 sensor constructs. (a) Melt temperatures for the SMN1 probes with SMN1 target (complement sequence) (i) and SMN2 target (ii) were determined using 3 nM solutions of each Cy5-labeled target in 1× TEN (1 M NaCl, 50 mM Tris–HCl, 10 mM EDTA, pH 7.0). (b) Melt temperatures for the immobilized SMN2 probes with SMN1A (iii) and SMN2A (iv) were determined using the procedure stated above.
