Table 2. Results from experiments measuring SMN1/SMN2 content in samples simulating clinical samples for SMA diagnosis.

Actual SMN1 aliquot	Measured SMN1 aliquot	Actual SMN2 aliquot	Measured SMN2 aliquot
0	0.03 ± 0.04 (n = 4)	2	2.4 ± 0.6 (n = 4)
1	1.2 ± 0.2 (n = 3)	3	2.6 ± 0.2 (n = 3)
2	2.6 ± 0.4 (n = 3)	2	2.6 ± 0.3 (n = 3)
3	3.4 ± 0.2 (n = 3)	1	1.2 ± 0.2 (n = 3)

Samples were prepared by mixing crude PCR mixtures that contained either SMN1 or SMN2, in various ratios. Each aliquot was 5 µl into a total final solution of 500 µl.