Table 3. Summary of statistical parameters for kinetic analysis of oligonucleotide target binding in the concentration range of 0.5–5 nM in 1× TEN at operating temparatures close to interfacial Tm (Tm – T < 3°C; n = 10).
Parameter |
y0 |
a |
b |
c |
d |
R2 (model fit) |
Maximum value | 1591 | 7484 | 0.2485 | 8396 | 0.01210 | 0.981 |
Minimum value | –64 | 433 | 0.0406 | 1051 | 0.00194 | 0.850 |
Mean | 282 | 3729 | 0.1537 | 2837 | 0.00508 | 0.920 |
RSD (%) | 175 | 58 | 41 | 77 | 62 | 6 |
Median | 72 | 3798 | 0.1443 | 2247 | 0.00385 | 0.935 |
Maximum and minimum values refer to the results of data analysis and do not necessarily correlate with sample concentration for all parameters.