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. 2013 Aug 5;8(8):e71136. doi: 10.1371/journal.pone.0071136

Figure 3. Molecular confirmation of transgenic Lines.

Figure 3

PCR amplification of (A) SbNHX1 gene (172 bp internal fragment), (B) hptII gene (963 bp) and (C) gus gene (157 bp internal fragment). Lane M is 100 bp Molecular weight marker ladder (Bangalore Genei, India); lane+is pCAMBIA1301-SbNHX1 (positive control). (D) Real time qPCR analysis to determine the ratio of gus to JcKASIII gene in transgenic lines. Three lines L2, L8 and L19 have a ratio of 0.74, 2.92 and 1.27, respectively. (E) Reverse transcriptase PCR of SbNHX1 transgenic lines. Amplification was observed in transgenic lines while there was no amplification from WT; JcKASIII gene was used as an internal control.