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. 2013 Aug 5;8(8):e71044. doi: 10.1371/journal.pone.0071044

Figure 1. Effect of tryptophan deficiency and kynurenine accumulation on Raw264.7 cell, fibroblast and Jurkat cell proliferation.

Figure 1

(A) Determination of the tryptophan deficiency effect on Raw264.7 cells proliferation and viability. Raw264.7cells were either cultured in RPMI, tryptophan-deficient medium (Trp-D) or tryptophan-deficient medium supplemented with 50 µg/ml tryptophan (Trp-D+Trp). MMT assay was done after 24 hours of treatment. (B) Evaluation of the kynurenine accumulation effect on Raw264.7 cells proliferation using MTT assay following 24 hours of treatment. (C) Evaluation of the effect of the tryptophan deficiency and high levels of kynurenine on Jurkat cells (solid bars) and dermal fibroblast (open bars) proliferation and viability using MTT assay after 24 hours of treatment. Data is mean±SEM of four independent experiments. (*P-value<0.05 and **P-value<0.01, n = 4).