Figure 2. The effect of tryptophan deficiency and high levels of kynurenine on Raw264.7 cell survival.

(A) Determination of cellular viability by FACs analysis using live/dead, viability/cytotoxicity assay kit. Raw264.7cells were either cultured in RPMI, tryptophan-deficient medium (Trp-D), tryptophan-deficient medium supplemented with 50 µg/ml tryptophan (Trp-D+Trp) or RPMI supplemented with 50 µg/ml kynurenine (RPMI+Kyn). The viability of Raw264.7 cells was determined by FACS analysis. (B) Raw264.7 cell survival is represented by the percentage of viable cells in the total cell population. Data is mean±SEM of three independent experiments (*P-value<0.05, n = 3).