Table 7.
ELISA assays for the detection of N. caninum in cattle bulk milk samples
| ELISA assay | Capture antigen | Sensitivity (95% confidence interval) | Specificity (95% confidence interval) | Reference |
|---|---|---|---|---|
| ISCOM ELISA (Boehringer Ingelheim Svanova, Uppsala Sweden) |
Tachyzoite antigen mixed with iscoms 1 |
50% |
81% |
[65] |
| (21-79%) |
(72-89%) |
|
||
| IDEXX Neospora antibody test |
Whole sonicated tachyzoites |
61% |
92% |
[66] |
| (49-73%) |
(87-98%) |
|
||
| LSI ELISA (Lissieu, France) |
Whole tachyzoite crude antigen lysate |
47% |
94% |
[66] |
| (35-60%) |
(90-99%) |
|
||
| Mastazyme® ELISA (Mast Diagnostics UK) |
Whole tachyzoites |
61-78% |
75-96% |
[67] |
| BioK 192/5, Jemelle, Belgium | Recombinant NcSRS2 protein | 95% | 96% | [7] |
1 Immunostimulatory complex composed of quillaja saponin, cholesterol and phospholipids.