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. Author manuscript; available in PMC: 2013 Aug 5.
Published in final edited form as: Science. 2012 Dec 20;339(6118):421–425. doi: 10.1126/science.1230473

Figure 3.

Figure 3

Content mixing assays with syntaxin-1-Munc18-1-liposomes and synaptobrevin-liposomes. (A) Traces showing the content mixing between syntaxin-1-Munc18-1 acceptor liposomes and synaptobrevin donor liposomes in the presence of SNAP-25, Munc13-1 C1C2BMUN (M13) and/or synaptotagmin-1 C2AB fragment (C2AB) (all in 0.5 mM Ca2+). The donor liposomes contained encapsulated self-quenched sulforhodamine and self-quenched DiD lipids. The y axis represents sulforhodamine fluorescence normalized to the maximum fluorescence observed upon detergent addition. (B) Quantification of the results obtained in the experiments of (B). (C,D) Traces showing the lipid mixing observed from DiD fluorescence de-quenching in the same experiments (C) and quantification of the results (D). Error bars represent standard deviations.